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Archiv-Übersicht     Angebot Nr. 12560

Angebotsdatum: 15. November 2017
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Biochemie
Titel des Themas: Characterisation of physiological and biophysical properties of adhesion G protein coupled receptor signaling

Institut: Rudolf Schönheimer Institute of Biochemistry
Adresse:
Prof. Tobias Langenhan
Johannisallee 30
04103 Leipzig
Tel.: +493419722100   Fax.:
Bundesland: Sachsen
Homepage: http://www.langenhan-lab.org
E-Mail Kontakt: mail

Beschreibung: Several fixed-term for 3 years PhD positions are open at the Langenhan Lab based at the University of Leipzig. The successful candidates will work at the interface between molecular pharmacology, physiology and biophysics in a project of the Rudolf Schönheimer Institute for Biochemistry.

The projects concern the investigation of novel class of surface receptor molecules that function as mechanosensors and are markers of cancer progression.

Central questions concern the fundamental physiological tasks, pharmacological principles and viscoelastic properties of these receptors and their expressing cells and tissues. The main objective is the determination how these receptors’ activation and signaling mechanism is implemented at the molecular and cellular scale. The projects will rest on the close collaboration between biomedical researchers, structural biologists and physicists. The experimental measurements will be facilitated by in-house theoretical and experimental support.

The successful candidates will establish structure-function studies and pharmacological signaling assays of receptor activity, and combine those – depending on the specific adhesion GPCR homolog – with (a) force measurements using atomic force microscopy-force spectroscopy and magnetic tweezers; (b) genetic and biofinformatical studies; or (c) physiological assays using Drosophila melanogaster as an in vivo model for adhesion GPCR signaling.

The PhD students will have access to state-of-the-art laboratories, which are located at the Institute of Biochemistry and partner institutions in Leipzig. The international and interdisciplinary environment at the department provides an excellent infrastructure allowing dynamic scientific exchange across the traditional disciplines. The lab has also a central role in the German (www.adhesiongpcr.de) and international adhesion GPCR community (www.adhesiongpcr.org) and successful candidates will gain immediate access to a young yet rapidly emerging field in biomedicine with substantial long-term large career potential.

The candidates should have a MSc. degree in physics, material science, biology or related fields, be fluent in English and have solid background in physics or molecular biology. Experience with the investigation of biomolecules with physical methods (e.g. AFM-FS, magnetic or optical tweezers), molecular biology or Drosophila is beneficial.

Leipzig University is an equal opportunity employer and is committed to increase the proportion of women in academia. Consequently, we actively encourage applications by female colleagues. Female candidates with equivalent qualifications and academic achievements will be preferentially considered within the framework of the legal possibilities. We also welcome applications from candidates with severe disabilities. Disabled candidates with equivalent qualifications will be preferentially considered.
Methoden: Molecular biology, Genetics, Drosophila melanogaster, CRISPR/Cas9, Atomic force microscopy-force spectroscopy, Confocal imaging, Super-resolution microscopy, Light-sheet microscopy, Protein biochemistry, Orthogonal protein labeling, Electrophysiology, Optogenetics
Anfangsdatum: 15. November 2017
Geschätzte Dauer: 3 Years
Bezahlung: TV-L-E13/65%
Papers: 1. Scholz, N., C. Guan, M. Nieberler, A. Grotemeyer, I. Maiellaro, S. Gao, S. Beck, M. Pawlak, M. Sauer, E. Asan, S. Rothemund, J. Winkler, S. Prömel, G. Nagel, T. Langenhan, and R. J. Kittel. 2017. Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons. elife 6.

2. Langenhan, T., X. Piao, and K. R. Monk. 2016. Adhesion G protein-coupled receptors in nervous system development and disease. Nat Rev Neurosci 17: 550–561.

3. Langenhan, T., M. M. Barr, M. R. Bruchas, J. Ewer, L. C. Griffith, I. Maiellaro, P. H. Taghert, B. H. White, and K. R. Monk. 2015. Model Organisms in G Protein-Coupled Receptor Research. Mol Pharmacol 88: 596–603.

4. Scholz, N., J. Gehring, C. Guan, D. Ljaschenko, R. Fischer, V. Lakshmanan, R. J. Kittel, and T. Langenhan. 2015. The Adhesion GPCR Latrophilin/CIRL Shapes Mechanosensation. Cell Reports 11: 866–874.

5. Langenhan, T., G. Aust, and J. Hamann. 2013. Sticky signaling - Adhesion class G protein-coupled receptors take the stage. Science signaling 6: re3.

6. Prömel, S., M. Frickenhaus, S. Hughes, L. Mestek, D. Staunton, A. Woollard, I. Vakonakis, T. Schöneberg, R. Schnabel, A. P. Russ, and T. Langenhan. 2012. The GPS Motif Is a Molecular Switch for Bimodal Activities of Adhesion Class G Protein-Coupled Receptors. Cell Reports 2: 321–331.

7. Langenhan, T., S. Prömel, L. Mestek, B. Esmaeili, H. Waller-Evans, C. Hennig, Y. Kohara, L. Avery, I. Vakonakis, R. Schnabel, and A. P. Russ. 2009. Latrophilin signaling links anterior-posterior tissue polarity and oriented cell divisions in the C. elegans embryo. Dev Cell 17: 494–504.
Sonstiges: Please submit your application by email with the usual documents (cover letter, CV, three references, Master’s transcript) before the December 31, 2017 to:

Prof. Tobias Langenhan, MD, DPhil (Oxon)
Faculty of Medicine
Rudolf Schönheimer Institute of Biochemistry
Division of General Biochemistry
Leipzig University
E: jobs@langenhan-lab.org

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