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Archiv-Übersicht     Angebot Nr. 12475

Angebotsdatum: 25. September 2017
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Biochemie
Titel des Themas: Small membrane proteins (SMPs) as organizers of the bacterial membrane

Institut: Institut für Biochemie und Molekularbiologie
Prof. Hans-Georg Koch
Stefan Meier Str 17
79104 Freiburg
Tel.: 0049-761-2035250   Fax.: 0049-761-2035289
Bundesland: Baden-Württemberg
Homepage: http://www.biochemie.uni-freiburg.de/ag/koch
E-Mail Kontakt: mail

Beschreibung: The prevalence and biological importance of small membrane proteins in all domains of life were only recently recognized by combing highly sensitive bioinformatics, genomics and proteomics approaches. Although the exact number of small proteins in the model organism Escherichia coli is still unknown, so far approx. 70 small proteins were experimentally verified of which approx. 25 are predicted to be membrane proteins. For only a few of these membrane proteins a putative function has been assigned, but data from E. coli and other bacteria suggest that SMPs are involved in two particular processes: (1) counteracting stress conditions (starvation, oxidative stress, temperature stress) and (2) organization and assembly of larger oligomeric complexes.

Employing a wide range of biochemical and biophysical methods we are analysing how small membrane proteins are targeted to and integrated into the cytoplasmic membrane of prokaryotic cells and how they influence membrane organisation and stress response. We are an international and highly motivated research team, working in a very attractive scientific environment and in one of the most beautiful parts of Germany.

We are looking for highly motivated applicants with a Master’s degree in life sciences and a strong background in Microbiology/Cell Biology and/or Biochemistry/Biophysics. Very good knowledge in English is required.

We are looking forward to receive your application including a motivation letter, your CV, records of your Bachelor and Master studies with the important certificates and two letters of reference via email
(hans-georg.koch@biochemie.uni-freiburg.de) until October 20th 2017. Informal inquiries about the position are welcome.

The position is funded by the German Science Foundation (DFG) as part of a national research initiative on small proteins in prokaryotes and will involve the collaboration with different research groups in Germany.
Methoden: in vitro transcription and translation systems
in vivo and in vitro site-directed cross-linking
fluorescence spectroscopy
membrane protein expression and purification
Anfangsdatum: 1. Dezember 2017
Geschätzte Dauer: 3 Jahre
Bezahlung: TV-L 13 (65%)
Papers: Kuhn, P., Draycheva, A., Vogt, A., Petriman, N.A., Sturm, L., Drepper, F., Warscheid, B., Wintermeyer, W., and Koch, H.G. (2015) Ribosome binding induces repositioning of the signal recognition particle receptor on the translocon. J. Cell Biol. 211, 91-104

David Braig, Tracy L. Nero, Hans-Georg Koch, Benedict Kaiser, Xiaowei Wang, Jan R. Thiele, Craig Morton, Johannes Zeller, Jurij Kiefer, Lawrence A.Potempa, Natalie A. Mellett, Luke A. Miles, Xiao-Jun Du, Peter J. Meikle, Markus Huber-Lang, G. Björn Stark, Michael W. Parker, Karlheinz Peter, Steffen U. Eisenhardt (2017) Transitional changes in the CRP structure lead to the exposure of pro-inflammatory binding sites. Nature communications 8, 14188

Denks, K., Sliwinski, N., Erichsen, V., Borodkina, B., Origi, A. and Koch, H.G. (2017) The signal recognition particle contacts uL23 and scans substrate translation inside the ribosomal tunnel Nature Microbiology, 2, 16265

Sachelaru, I., Winter, L., Knyazev, D., Zimmermann, M., Vogt, A., Kuttner, R., Ollinger, N., Siligan, C., Pohl, P. and Koch, H.G. (2017) YidC and SecYEG form a heterotetrameric protein translocation channel. Scientific reports 7, 101